Sara Semperboni ha ottenuto il diploma di maturità scientifica nel 2006 presso il Liceo Scientifico Andrea Fantoni di Clusone (BG). Ha conseguito la laurea triennale in Biotecnologie nel 2011 e la laurea specialistica in Biotecnologie Mediche nel 2014 presso l’Università degli Studi di Milano Bicocca (Monza).
Dal 2014 è coinvolta in studi preclinici in vitro ed in vivo di neurotossicità periferica da chemioterapici presso l’Unità di Neurologia Sperimentale dell’Università degli Studi di Milano Bicocca dove attualmente svolge il Dottorato di Ricerca in Neuroscienze.
Si è occupata principalmente di meccanismi molecolari in cellule tumorali e nervose, di tecniche di biologia molecolare e di utilizzo di modelli animali sperimentali di neurotossicità.
PROGETTO DI RICERCA
The role of Organic Cation Transporters 1 and 2 in Platinum drugs-induced peripheral neurotoxicity
- Curriculum: Neuroscienze Sperimentali
- Tutor: Paola Marmiroli
Cisplatin (CDDP) is a chemotherapeutic drug widely employed in the treatment of various solid tumors but its use is associated with severe side effects such as nephro-, oto- and neurotoxicity.
CDDP is a hydrophilic drug that can cross the plasma membrane and reach its targets also through membrane transporters. In this study we focused on the Organic Cation Transporters (OCT) 1 and 2 because of their specific interaction with CDDP and their specific organ distribution that may explain CDDP selective toxicity. Many in vivo studies have shown that OCTs expression in tumor cells is down-regulated and so the inhibition of OCT may be an attractive target to reduce CDDP toxicity, without impairing its antitumor efficacy.
We are investigating the role of OCT2 in CDDP induced neurotoxicity in vivo using OCT2 and OCT1,2 knockout (OCT2-/-, OCT1,2-/-) mice with a C57BL6 and FVB background respectively. We are also studying a possible neurotoxicity protection due to OCT2 and OCT1,2 KO in vitro, analyzing the cell survival of DRG neurons primary culture obtained from male and female mice, WT, OCT2-/- and OCT1,2-/- KO.
PUBBLICAZIONI E PARTECIPAZIONI A CONGRESSI RECENTI
Congressi
- Semperboni S., Chiorazzi A., Ceresa C., Canta A., Nicolini G., Cavaletti G., Marmiroli P. “OCT2 transporter involvement in development of cisplatin toxicity”. XXIII Convegno Annuale A.I.S.A.L.. 10-12 ottobre 2016, Roma, Italia.
- Ceresa, G. Nicolini, S. Semperboni, M. Pellei, N. Marigotta, V. Gandin, J. Hoeschele, C. santini, G. Cavaletti. “ In vitro activity and neurotoxicity of new promising metal-based anticancer complexes”. Second international meeting of the Milan centre for neuroscience. July 6-8, 2016, Milan, Italy.
- Sara Semperboni, Valentina Carozzi, Beatrice Riva, Eleonora Pozzi, Giulia Fumagalli, Guido Cavaletti, Armando Genazzani, Paola Marmiroli. “The severity of oxaliplatin neurotoxicity is related to differential expression of a solute transporter in mice”. European Meeting of Neuroscience by Ph.D. Student, 2nd Edition, April, 29th 2016, Grenoble, France.
ALTRE INFO
Per la lista completa delle pubblicazioni visita ORCID
