CURRICULUM VITAE
Ilenia Savinetti, nata a Milano il 19/09/1992, ha conseguito la Laurea Triennale in Biotecnologie a novembre 2015 c/o l’Università Degli Studi di Milano-Bicocca e nel 2018 la Laurea Magistrale in Neurobiologia cum laude c/o L’università Degli Studi di Pavia. Durante il periodo di tesi magistrale, ambito di Ricerca è stato il ruolo dell’Immunità Innata, in particolare dei monociti, in pazienti affetti da Sclerosi Multipla.
Da marzo 2018 ad ottobre 2018, Visiting Researcher c/o il Laboratorio di Immunologia Cellulare e Molecolare della Dott.ssa Maria Foti – Università Degli Studi di Milano-Bicocca.
Da Novembre 2018 è Dottoranda in Neuroscienze Sperimentali c/o l’Università Degli Studi di Milano-Bicocca, sotto la supervisione della Dott.ssa Maria Foti, laboratorio nel quale sta proseguendo il suo progetto di tesi magistrale.
PROGETTO DI RICERCA
Gene Expression Profiling Identifies Specific Signatures in Peripheral Blood Monocytes of Primary Progressive Multiple Sclerosis Patients.
- Curriculum: Neuroscienze Sperimentali
- Tutor: Maria Foti
Multiple Sclerosis is an autoimmune disease that affects the Central Nervous System (CNS) leading to demyelination, axonal and neurological damage, often affecting young adults. MS is considered to be a T cell mediated disease but in recent years different studies have shown that monocytes and monocyte-derived macrophages are also crucial for the development of MS. In this project we aimed to investigate the contribution of the innate immune system in the MS pathology. Peripheral blood monocytes from three groups of individuals: healthy controls, RRMS and PPMS patients have been studied by using a Transcriptomics approach. With this technology, we were able to identify a number of dysregulated pathways specifically induced in monocytes of Primary Progressive Patients (PP) compared to Relapsing Remitting (RR) patients and Healthy controls. We focus our analysis on specific metabolic changes observed in PP-derived monocytes and in a subgroup of RR-monocytes. These pathways will be studied on monocytes mRNA by qRT-PCR and their protein level by ELISA assay on sera of patients. Gene expression analysis will be focused on modulated receptors identified by transcriptomics analysis. The receptors complex will be validated by qRT-PCR in PPMS monocytes and the receptor regulation will be studied in ex vivo monocytes. To understand which cellular types within the brain and/or the periphery will be affected by the monocytes activation state, a co-culture systems of activated monocytes with purchased primary human microglia, astrocytes, oligodendrocytes and neurons will be established and studied by FACS analysis and functional assays.
PARTECIPAZIONE A CONGRESSI
- BraYn Conference (2nd Brainstorming Research Assembly for Young Neuroscientists), Milano, 14-16 Novembre 2019.
- SIICA (XII National Congress), 26-28 Maggio 2021.
PUBBLICAZIONI
Savinetti, I.; Papagna, A.; Foti, M. Human Monocytes Plasticity in Neurodegeneration. Biomedicines 2021, 9, 717.
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